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| Registros recuperados : 11 | |
| 3. |  | FRANZIN, A.; MARUYAMA, S.; SINGH, M.; MORE, D.; FERREIRA, B.; RIBEIRO, J.; BISHOP, R.; SANTOS, I. K. F. de M. A systems biology approach for developing an anti-tick vaccine for cattle. In: INTERNATIONAL VETERINARY VACCINES AND DIAGNOSTICS CONFERENCE, 5, 2009, Madison, WI USA. [Proceedings...]. Madison: [s.n.], 2009.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 4. | | ABATEPAULO, A. R. R.; CAETANO, A. R.; SANTOS, I. K. F. de M.; MORÉ, D. D.; CARVALHO, W. A.; SILVA, J. S. da. Detection of single nucleotide polymorphisms (SNPs) in bovine immune-response candidate genes for mediating resistance to infestations with the cattle tick, Rhipicephalus (Boophilus) microplus. In: INTERNATIONAL CONFERENCE ON ANIMAL GENETICS, 30., 2006, Porto Seguro, BA. [Proceedings...]. Belo Horizonte, MG: CBRA, 2006. Não paginado.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 5. | | MARUYAMA, S. R.; HORACKOVA, J.; TEIXEIRA, F. R.; MORE, D. D.; GARCIA, G. R.; FERREIRA, B. R.; SANTOS, I. K. F. de M. Reverse immunogenomics for discovery of new antigens for a vaccine against the cattle tick, Rhipicephalus microplus. In: EUROPEAN VETERINARY IMMUNOLOGY WORKSHOP, 3, 2009, Berlin. Abstract Book of ECI. [s. l.]: European Veterinary Immunology Group, 2009.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | CARVALHO, W. A.; BECHARA, G. H.; MORÉ, D. D.; FERREIRA, B. R.; SILVA, J. S. da; SANTOS, I. K. F. de M. S. Rhipicephalus (Boophilus) microplus: distinct acute phase proteins vary during infestations according to the genetic composition of the bovine hosts, Bos taurus and Bos indicus. Experimental Parasitology, v. 118, p. 587-591, 2008.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | SOUZA, M. M.; MOKRY, F. C.; TIZIOTO, P. C.; OLIVEIRA, P. S. N.; SOMAVILLA, A.; CESAR, A. S. M.; MORÉ, D.; MOURÃO, G.; DINIZ, W. J. S.; MUDADU, M. A.; NICIURA, S. C. M.; ZERLOTINI, A.; REGITANO, L. C. Allele specific expression analysis in bovine muscle tissue. In: INTERNATIONAL CONFERENCE OF TH AB3C, 11.; BRAZILIAN SYMPOSIUM OF BIOINFORMATICS, 2015, São Paulo. Proceedings... São Paulo: USP-São Paulo, 2015.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 8. | | MORÉ, D. D.; MALAGO JUNIOR, W.; MUDADU, M. de A.; ZERLOTINI NETO, A.; GULIAS-GOMES, C. C.; IBELLI, A. M. G.; CATOIA, V.; CARDOSO, F. F.; REGITANO, L. C. de A. Genetics mechanisms of resistance and response to tick infestation in Hereford cattle: a global view. In: INTERNATIONAL SYMPOSIUM ON ANIMAL FUNCTIONAL GENOMICS, 5., 2013, Guarujá. Abstract... Guarujá:[ s.n.], 2013. AB.59.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Pecuária Sudeste; Embrapa Pecuária Sul. |
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| 9. | | MORÉ, D. D.; CARDOSO, F. F.; MUDADU, M. de A.; MALAGO JUNIOR, W.; GULIAS GOMES, C. C.; SOLLERO, B. P.; IBELLI, A. M. G.; COUTINHO, L. L.; REGITANO, L. C. de A. Network analysis uncovers putative genes affecting resistance to tick infestation in Braford cattle skin. BMC Genomics, v. 20, n. 998, 2019.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Pecuária Sudeste; Embrapa Pecuária Sul; Embrapa Suínos e Aves. |
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| 10. | | SOUZA, M. M.; MOKRY, F. C.; TIZIOTO, P. C.; OLIVEIRA, P. S. N.; SOMAVILLA, A.; CESAR, A. S. M.; MORÉ, D.; MOURÃO, G.; DINIZ, W. J. S.; MUDADU, M. A.; NICIURA, S. C. M.; COUTINHO, L. L.; ZERLOTINI NETO, A.; REGITANO, L. C. Allele specific expression analysis in bovine muscle tissue. In: ANNUAL INTERNACIONAL CONFERENCE ON INTELLIGENT SYSTEMS FOR MOLECULAR BIOLOGY, 23.; EUROPEAN CONFERENCE ON COMPUTATIONAL BIOLOGY, 14., 2015, Dublin. Posters... [S.l.]: International Society for Computacional Biology, [2015]. Não paginado. ISMB/ECCB 2015. Pôster D17.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 11. | | SOUZA, M. M.; MOKRY, F. C.; TIZIOTO, P. C.; OLIVEIRA, P. S. N.; SOMAVILLA, A.; CESAR, A. S. M.; MORÉ, D.; MOURÃO, G.; DINIZ, W. J. S.; MUDADU, M. A.; NICIURA, S. C. M.; COUTINHO, L. L.; ZERLOTINI, A.; REGITANO, L. C. Allele specific expression analysis in bovine muscle tissue. In: INTERNATIONAL CONFERENCE OF THE AB3C, 11.; BRAZILIAN SYMPOSIUM OF BIOINFORMATICS, 2015, São Paulo. Proceedings... [S.l.]: AB3C, 2015. p. 197. X-meeting 2015.| Biblioteca(s): Embrapa Agricultura Digital. |
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| Registros recuperados : 11 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Pecuária Sudeste; Embrapa Pecuária Sul; Embrapa Suínos e Aves. |
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Data corrente: |
03/01/2020 |
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Data da última atualização: |
03/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MORÉ, D. D.; CARDOSO, F. F.; MUDADU, M. de A.; MALAGO JUNIOR, W.; GULIAS GOMES, C. C.; SOLLERO, B. P.; IBELLI, A. M. G.; COUTINHO, L. L.; REGITANO, L. C. de A. |
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Afiliação: |
Daniela D. Moré, CPPSE; FERNANDO FLORES CARDOSO, CPPSUL; MAURICIO DE ALVARENGA MUDADU, CNPTIA; WILSON MALAGO JUNIOR, CPPSE; CLAUDIA CRISTINA GULIAS GOMES, CPPSUL; BRUNA PENA SOLLERO, CPPSUL; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; Luiz L. Coutinho, USP; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
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Título: |
Network analysis uncovers putative genes affecting resistance to tick infestation in Braford cattle skin. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
BMC Genomics, v. 20, n. 998, 2019. |
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DOI: |
10.1186/s12864-019-6360-3 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Genetic resistance in cattle is considered a suitable way to control tick burden and its consequent losses for livestock production. Exploring tick-resistant (R) and tick-susceptible (S) hosts, we investigated the genetic mechanisms underlying the variation of Braford resistance to tick infestation. Skin biopsies from four-times-artificially infested R (n = 20) and S (n = 19) hosts, obtained before the first and 24 h after the fourth tick infestation were submitted to RNA-Sequencing. Differential gene expression, functional enrichment, and network analysis were performed to identify genetic pathways and transcription factors (TFs) affecting host resistance. Results: Intergroup comparisons of hosts before (Rpre vs. Spre) and after (Rpost vs. Spost) tick infestation found 51 differentially expressed genes (DEGs), of which almost all presented high variation (TopDEGs), and 38 were redundant genes. Gene expression was consistently different between R and S hosts, suggesting the existence of specific anti-tick mechanisms. In the intragroup comparisons, Rpost vs. Rpre and Spost vs. Spre, we found more than two thousand DEGs in response to tick infestation in both resistance groups. Redundant and non-redundant TopDEGs with potential anti-tick functions suggested a role in the development of different levels of resistance within the same breed. Leukocyte chemotaxis was over-represented in both hosts, whereas skin degradation and remodeling were only found in TopDEGs from R hosts. Also, these genes indicated the participation of cytokines, such as IL6 and IL22, and the activation of Wingless (WNT)-signaling pathway. A central gene of this pathway, WNT7A, was consistently modulated when hosts were compared. Moreover, the findings based on a genome-wide association study (GWAS) corroborate the prediction of the WNT-signaling pathway as a candidate mechanism of resistance. The regulation of immune response was the most relevant pathway predicted for S hosts. Members of Ap1 and NF-kB families were the most relevant TFs predicted for R and S, respectively. Conclusion: This work provides indications of genetic mechanisms presented by Braford cattle with different levels of resistance in response to tick infestation, contributing to the search of candidate genes for tick resistance in bovine. MenosBackground: Genetic resistance in cattle is considered a suitable way to control tick burden and its consequent losses for livestock production. Exploring tick-resistant (R) and tick-susceptible (S) hosts, we investigated the genetic mechanisms underlying the variation of Braford resistance to tick infestation. Skin biopsies from four-times-artificially infested R (n = 20) and S (n = 19) hosts, obtained before the first and 24 h after the fourth tick infestation were submitted to RNA-Sequencing. Differential gene expression, functional enrichment, and network analysis were performed to identify genetic pathways and transcription factors (TFs) affecting host resistance. Results: Intergroup comparisons of hosts before (Rpre vs. Spre) and after (Rpost vs. Spost) tick infestation found 51 differentially expressed genes (DEGs), of which almost all presented high variation (TopDEGs), and 38 were redundant genes. Gene expression was consistently different between R and S hosts, suggesting the existence of specific anti-tick mechanisms. In the intragroup comparisons, Rpost vs. Rpre and Spost vs. Spre, we found more than two thousand DEGs in response to tick infestation in both resistance groups. Redundant and non-redundant TopDEGs with potential anti-tick functions suggested a role in the development of different levels of resistance within the same breed. Leukocyte chemotaxis was over-represented in both hosts, whereas skin degradation and remodeling were only found in TopDEGs from... Mostrar Tudo |
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Palavras-Chave: |
Bovine; Braford; Enrichment analysis; Expressão gênica; Gado Braford; Network analysis; RNA-Seq. |
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Thesagro: |
Bovino; Carrapato; Resistência; Resistência Genética. |
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Thesaurus NAL: |
Cattle; Gene expression; Rhipicephalus microplus; Tick infestations. |
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Categoria do assunto: |
--
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208137/1/More-et-al-2019.pdf
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Marc: |
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001 2118135
005 2020-01-03
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1186/s12864-019-6360-3$2DOI
100 1 $aMORÉ, D. D.
245 $aNetwork analysis uncovers putative genes affecting resistance to tick infestation in Braford cattle skin.$h[electronic resource]
260 $c2019
520 $aBackground: Genetic resistance in cattle is considered a suitable way to control tick burden and its consequent losses for livestock production. Exploring tick-resistant (R) and tick-susceptible (S) hosts, we investigated the genetic mechanisms underlying the variation of Braford resistance to tick infestation. Skin biopsies from four-times-artificially infested R (n = 20) and S (n = 19) hosts, obtained before the first and 24 h after the fourth tick infestation were submitted to RNA-Sequencing. Differential gene expression, functional enrichment, and network analysis were performed to identify genetic pathways and transcription factors (TFs) affecting host resistance. Results: Intergroup comparisons of hosts before (Rpre vs. Spre) and after (Rpost vs. Spost) tick infestation found 51 differentially expressed genes (DEGs), of which almost all presented high variation (TopDEGs), and 38 were redundant genes. Gene expression was consistently different between R and S hosts, suggesting the existence of specific anti-tick mechanisms. In the intragroup comparisons, Rpost vs. Rpre and Spost vs. Spre, we found more than two thousand DEGs in response to tick infestation in both resistance groups. Redundant and non-redundant TopDEGs with potential anti-tick functions suggested a role in the development of different levels of resistance within the same breed. Leukocyte chemotaxis was over-represented in both hosts, whereas skin degradation and remodeling were only found in TopDEGs from R hosts. Also, these genes indicated the participation of cytokines, such as IL6 and IL22, and the activation of Wingless (WNT)-signaling pathway. A central gene of this pathway, WNT7A, was consistently modulated when hosts were compared. Moreover, the findings based on a genome-wide association study (GWAS) corroborate the prediction of the WNT-signaling pathway as a candidate mechanism of resistance. The regulation of immune response was the most relevant pathway predicted for S hosts. Members of Ap1 and NF-kB families were the most relevant TFs predicted for R and S, respectively. Conclusion: This work provides indications of genetic mechanisms presented by Braford cattle with different levels of resistance in response to tick infestation, contributing to the search of candidate genes for tick resistance in bovine.
650 $aCattle
650 $aGene expression
650 $aRhipicephalus microplus
650 $aTick infestations
650 $aBovino
650 $aCarrapato
650 $aResistência
650 $aResistência Genética
653 $aBovine
653 $aBraford
653 $aEnrichment analysis
653 $aExpressão gênica
653 $aGado Braford
653 $aNetwork analysis
653 $aRNA-Seq
700 1 $aCARDOSO, F. F.
700 1 $aMUDADU, M. de A.
700 1 $aMALAGO JUNIOR, W.
700 1 $aGULIAS GOMES, C. C.
700 1 $aSOLLERO, B. P.
700 1 $aIBELLI, A. M. G.
700 1 $aCOUTINHO, L. L.
700 1 $aREGITANO, L. C. de A.
773 $tBMC Genomics$gv. 20, n. 998, 2019.
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Embrapa Pecuária Sul (CPPSUL) |
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